KAUST researchers are working to improve gene editing tools, specifically CRISPR/Cas9, for crop bioengineering to address food security challenges. Magdy Mahfouz's lab is developing a germline engineering platform to produce gene-edited plants without foreign DNA and bypass time-consuming tissue culture. A recent European court decision classifies CRISPR/Cas9 crops as GMOs, facing stringent regulations, contrasting with the U.S. where CRISPR-edited mushrooms are already available. Why it matters: Advances in gene editing at KAUST could significantly enhance crop yields and stress tolerance in the region, but regulatory hurdles remain a key challenge for deployment.
A KAUST team developed piRNAi, a gene-silencing tool in nematode worms using synthetic RNA sequences interacting with the piRNA pathway. They successfully silenced genes involved in sex determination and other functions, demonstrating multiplexed gene silencing. The gene silencing lasted for varying durations across generations, up to six generations. Why it matters: This expands the molecular toolkit for gene manipulation and offers potential therapeutic applications in humans, given the presence of the same gene-silencing pathway.
KAUST plant scientists are advocating for the deployment of new plant breeding technologies, including gene editing, to enhance global food security. Researchers Mark Tester and Magdy Mahfouz highlight these methods' potential to improve crops by minimizing crop life cycle for research on breeding, selection, and fixing of useful genes. They argue these technologies offer alternatives to genetically modified crops, potentially lowering regulatory costs and increasing seed affordability for farmers in developing countries. Why it matters: These advancements, coupled with regional seed-sharing initiatives, could significantly boost food production and accessibility in less-developed countries in the Middle East and globally.
Juan Carlos Izpisua Belmonte from the Salk Institute discussed aging and regenerative medicine at the KAUST 2019 Winter Enrichment Program. His team is combining gene editing and stem cell technologies to grow rat organs in mice and human cells in pig and cattle embryos. The Salk team is collaborating with KAUST to rejuvenate organs using noncoding RNAs and small metabolites. Why it matters: This research collaboration between KAUST and the Salk Institute explores innovative approaches to address age-related diseases and organ regeneration, with potential long-term impacts on healthcare in the region.
Khaled Alsayegh at the King Abdullah International Medical Research Center is creating a Saudi Stem Cell Donor Registry, with 80,000 potential donors identified. The aim is to identify universal donors, reprogram their cells into induced pluripotent stem (iPS) cells, and create a gene bank for matched tissue transplants. Alsayegh is collaborating with Jesper Tegnér at KAUST to create pacemaker cells using single-cell RNA sequencing. Why it matters: This initiative could revolutionize precision medicine in KSA by providing readily available, matched cells for transplants, reducing the need for patient-specific reprogramming and improving treatment outcomes.
KAUST and KFSHRC have developed NanoRanger, a new gene sequencing system for identifying mutations causing genetic diseases. NanoRanger offers a faster and simpler process to detect DNA abnormalities at base resolution, building on existing long-read sequencing technologies. The system is designed to be cheaper and faster, targeting diseases prevalent in Saudi Arabia due to consanguinity. Why it matters: The technology has the potential to improve diagnosis and treatment of Mendelian diseases, which are especially prevalent in the Arab world.
Researchers at KAUST and Peking University Third Hospital have created a novel blastoid model for studying early human development using extended pluripotent stem cells (EPSCs). The blastoid is a 3D cell model mimicking the blastocyst phase, avoiding ethical concerns associated with using human embryos. The team showed that blastoids can be cultured to mimic post-implantation development, offering insights into early cell lineages. Why it matters: This innovation provides a way to study human embryogenesis without the ethical constraints of using actual embryos, potentially advancing our understanding of miscarriage and birth defects.
Dr. John Bedbrook of DiCE Molecules LLC spoke at KAUST about the challenges of feeding a growing population with increasingly stressed arable land. He noted the increasing demand for meat in emerging economies exacerbates the problem. Bedbrook emphasized the role of genetics and hybridization in improving crop yields and quality to address food security. Why it matters: Investments in agricultural biotechnology are crucial for the GCC region to enhance food security and reduce reliance on imports amid changing climate conditions.